EGFP expression in the forebrain (3rd ventricle), midbrain (floor plate, roof plate), hindbrain (floor plate, ventricle), spinal cord (floor plate, roof plate), eyes, lateral line and ear.

Genomic location: Chromosome 14

The insertion is in the intron of proml1 (Prominin-like 1)

DNA sequence flanking Tol2 insertion site

5:TTCATGAGTGAATGTGAAActgccgaactgcAGTTAAAGTCACCCAAAAT

TACAGGAAACTCTTACATGAAAGCACTTCATGTAAACACCTTAAAGGTGCAG

TAGGTGATCGTCTTCAAAAGCATGTTTTGTTGTGCTGGTTGAAAGTCTCTTC

ATATTCCAATAGTAATGATTACAGTAAATGATCTAAATGTGTTATATGTATT

TTTATATTTTGGGTACAGCACAACACTAAAAAGTGTTCATCCAATTAAATAT

TGTCAAACCGACATCTCCCATATTTCCAATAAGCAGC